I really need someone to start me off with my practical questions. 

1. Buffer will be 100ml of 0.25 M Tris buffer with a ph of 8.0. Write out the chemical equation for this buffer. Identify which species is the conjugate acid and conjugate base.

2. For this buffer, calculate the ratio of the base to acid using the Henderson-Hasselbach equation. 

 

I don't really know where to begin to be honest. Any help/ info would be so appreciated!

58 minutes ago, plk21 said:

I don't really know where to begin to be honest.

This is coursework/homework and belongs in that section.

You are told which equation to use in part (2) so I sugest you start by writing the HH equation down and trying to indentify the species involved.

That should give you a big clue.

Here is another one.

https://www.aatbio.com/resources/buffer-preparations-and-recipes/tris-buffer

 

When you have some detail to ask about, come back for more help.

!

Moderator Note

Moved to Homework Help.