Hi,

 

I need a proper direction.

I am conducting an independent experiment.

The aim is to put an ectA gene into the yeast.

It seems that I cannot get an ectABC operon from anywhere, however SigmaAldrich has ectoine for sale.

 

So,

 

I am having a protein - but I need to get a gene of interest.

In the terms of experiment, how do I go from there?

 

Do I need to construct a cDNA?

 

Regards,

I.

You can't get it made? I had thought there were companies that did this, though I couldn't tell you which ones.

I've tried to find it anywhere- but I can't. The companies sell only the protein - ectoine. But I am interested in the particular gene - ectA gene.

 

So if I have a protein, do I create a cDNA library and then use primers to find a specific gene of interest?

There are companies that sell custom genes, like Genscript or Biomatik. Have you tried them?

Alternatively, if you know from which organism you want it, just get it (or its DNA) from a culture bank, get the right primers and amplify it.