Dear all,

 

Recently, I am performing a cultivation experiment that requires large volume of medium per sample.

 

However, I am concerned about whether cultivation medium loss will affect experimental results or not.

 

I'm not sure which strategy is better to reduce(minimize sampling loss) or increase(maximze data pont) sampling number.

 

If you have any idea or related paper, please help me.

 

Thank you.

Please expand your question to describe your experiment more fully as you have posted this in engineering.

 

What are you cultivating?

 

What is the cultivating medium?

 

For instance is it solid(eg agriculture) or liquid (eg a blood culture)?

 

You could review this article on blood for the statistics content.

 

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4996091/

 

A description of the quantities involved would be helpful; there are recognized engineering techniques for dealing with large volumes of solid medium, such as soil, to be sampled such as the riffle box.

Edited October 27, 20169 yr by studiot

The question is cultivation of microalgae using BG11 medium.

 

BG11 is a liquid medium for fresh microalgae.

 

Analysis of lipid contents in microalgae requires too much volume of culture medium...

It depends what you mean with medium loss. Do you mean loss via evaporation? Or due to sampling? Is it a time-course? It also depends whether the effects result in systematic changes. In case of doubt, doing more biological replicates can address the issue to some degree, as it would tell you a bit about the reproducibility of the procedure.

Edited November 2, 20169 yr by CharonY