Genetics

hello to everyone i need some help about my research i have been trying to obtain triple mutant in saccharomyces cerevisiae lacking all three pyruvate decarboxylase genes but i cannot get colonies incomplex media having glucose as carbon source..i am using the LiAc/PEG/SS DNA protocol ...till now i am successful to obtain double mutant but not triple mutan...i need really your help because my work depends on that triple mutant..Thanks for your help

we know that a genetic code transfer from one generation to another then i wan to ask is there anythng like that ke i person also trasfer its life time event memores to its offspring if yes then is it possible to see the past from this genetic coding

Trying to submit a novel deleterious mutation to HGVS but both ends are in intergenic sequence. How on earth do I name it in their nomenclature??

Hello i was wondering how is it possible for bacteria to gain genes responsible for antibiotic resistance , after all, it is obvious that bacteria can not produce new genetic information just because there is an antibiotic in environment .

Folks: The subject line says it all. Roughly how many atoms (10^6, 10^9, or larger) constitute one human T cell? I believe the size varies from 5 to 10 microns. That's not helpful to me. Thanks to all. GLWD

Hello all, I am starting a project and have some questions that I hope someone may answer. 1) What is the gene(s) that signal for the production of a)Fructose, b)Sucrose and c)Dextrose in fruiting plants? 2) Are they the same gene(s) in a variety of fruiting plants or do they differ (probably differ, but just want to confirm. Thanks in advance for your valuable input. JQ.

This question has been festering in my brain for a few years now. The idea that evolution in humans took a misstep. I wonder does evolution (Explained by Charles Darwin and Alfred Russel Wallace ) have something like a logic error in the programming of genes? The survival of a species relies on the weak mutations to die out and strong mutations to survive and breed, but, what if a mutation occurs (like that at the divergence from Homo Erectus to Homo Sapiens and Homo Sapien Neanderthalensis) that allows the specimen with weak genes to be supported by the one with strong? I don't mean anything ethical racial just the Homo Sapien Sapien as a race when I say weak. The key…

I understand the sex chromosomes and the significance of the extra X making the 47xxy karyotype. but I don't understand why the 1st set of 22 autosomes are divided into 1 and 21. Can you help me? I'm doing a report on Klinefelter syndrome for nursing school and I found this graphic but it confused me on the autosomes.

I know that when two variants are close together and you've done an association study, both can come up as showing evidence for association just because they are in close proximity. I read that conditional association analysis can account for the effect of one variant on the other. How is conditional analysis done, and what value tells you whether or not the variants are independent of one another? Does the odds ratio tell you? If so, what value should it be?

When a population comes through a bottleneck which results in a drastically decreased variability within the gene pool, does the variability recover through mutations? How fast? Humans generally are highly inbred - I remember reading somewhere that two chimpanzees from the same forest may differ more from each other than the furthest removed human populations. There is also a lot of variation between various human groups - Africans are generally the most diverse and East Asians the least. Ashkenazi Jews are so inbred that they are basically all related to each other as fourth or fifth degree cousins. Cheetahs are so inbred that skin can be transplanted between in…

I have a friend who asked me a weird question about science the other day. I'll try to explain this the best I can, as I know little about Genetics. Claim 1: People develop genetic disorders like Hunting's disease because two halves of one gene. Example, two people have sex and both have half the copy of Hunting's, but don't suffer from Hunting's; the chances of the baby having Hunting's is more likely to occur. Claim 2: Having incest sex can lead to a higher risk of genetic disorders; due to the engaging parties having almost the same genetics. Question: If Claim 1 is true, then is it possible as the population rises; the risk of genetic disorders are greater…

I got confused while reading a footnote by Dawkins in his book The Selfish Gene (which I so far thoroughly enjoy), elaborating upon why incestuous relations are highly detrimintal to offspring genetically. Here is the full note: “A lethal gene is one that kills its possessor. A recessive lethal, like any recessive gene, doesn't exert its effect unless it is in double dose. Recessive lethals get by in the gene pool, because most individuals possessing them have only one copy and therefore never suffer the effects. Any given lethal is rare, because if it ever gets common it meets copies of itself and kills off its carriers. There could nevertheless be lots of different …

Hello. I am a 19 yr male university student, coming from Central/Eastern Europe. I am not a stupid person, my intelligence is above average (I'd estimate it at about 105-110), my parents are both high school graduates (high school in Central Europe is tougher than in US/UK) , my mother has a master's degree (few people in communist Poland pursued higher education), my sister has a M.A in law and works in law enforcement.However I'm not an extremely smart person either. Since 7th grade (the subjects of physics and chemistry are introduced in 7th grade here) my strongest area of science was chemistry and I also liked it the most, then math, then physics. However, I have so…

I am trying to understand if a chromosome is made of one DNA molecule or just a part of a DNA molecule. Maybe i am confused with the DNA molecule itself. The way it is taught seems to indicate that each human beings has DNA molecules that are all the same. Then how can the same molecule make different chromosome with different size?

Hi, I need quick help on understanding transcription and translation. I think I know what transcription is and maybe translation is but here is what I think they are: In transcription, DNA's replication that in which RNA involves. This means that instead of DNA replicating the same thing, RNA gets involved and RNA is made, which has antisequence of DNA. Suppose DNA is ACG then RNA is UGC. By the way, thymine is replaced with uracil in RNA. In translation, decoding RNA messages into polypeptide chain, or simply, rewriting in protein. But my question is, what does transfer RNA do? I know that messenger RNA carries out the information for DNA around the cell …

Dear all, It is important to keep abreast of the continuous development of NGS and array software and avoid redundancy. [Link removed] provides an overview of more than 2200 tools. All tools have been classified and detailed information provided. By making this resource available, we aim to help both bioinformatics experts and other researchers find appropriate tools for their needs. The directory can also be used by life scientists as an educational or quick-reference support. If you find any missing software, do not hesitate to tell me! Arnaud

Hi! I am looking for peer-reviewed sources (e.g., scholarly articles) about genes associated with ethnic culture, if any. Thank you.

Hello, As an amateur, i'd like to ask a very few and basic questions about genetics : 1- Can the genes be altered through a human's life or are they immutable from the beginning till death ? 2- At what scale is it already possible to alter human genes ? 3- How Does altering human genes work, is it very simple or a long operation and how much time do the first results expected from gene manipulation start to show up ? 4- If the genes in a human being are replicated millions of times in every cell, how does one modify genes, does he modify only one piece of DNA or does he have to modify every piece of dna of the human body, or is there a part of the body that is …

Hello, I am looling for some information regarding pre-natal DNA paternity testing. This testing hasn't been around forever and I would like to understand it a bit more. Please consider the following scenario. The sampling method was Bucall cells from father and blood from mother. "DNA was isolated from the buffy-coat samples of the mother and the buccal cells of the alleged father. DNA was sequenced at numerous single nucleotide polymorphisms (SNPs) located on multiple chromosomes throughout the human genome. The plasma DNA, which contains both fetal DNA and maternal DNA, was analyzed at the thirty two (32) informative SNPs." "Among the thirty two (32) informative SN…

I have calculated individual inbreeding coefficients in PLINK software ( --het) and took the average of all individuals from a population to calculate inbreeding in the different populations. These coefficients are based on loss of heterozygosity (i.e. based on observed versus expected number of homozygous genotypes). Now, I have calulated observed and expected heterozygosity (Ho and He), also in PLINK software (--hardy), and for each population Ho is larger than He while in the table of individual inbreeding coefficients the number of observed homozygous genotypes is larger than the number of expected genotypes. So, this would mean that there are more heterozygous indiv…

While working in the Hyperbaric unit of a hospital near LA in the early 1980's a first aide instructor from the Navy told us that the Mojave green rattlesnake was produced by the cross breading experiments carried out by the military during the 1950's and 60's. Can anyone please set the record straight, as I can not locate any data to backup this claim.

Dear all, I just found this great place to discuss science! I was wondering if anyone has an idea that roughly how many Drosophila genes have a phenotype when mutated? I think I have read it somewhere that only 1/3 of the genes would give a phenotype but am not entirely sure about that. Could anyone please help me out? Thank you

People, Suspect another scam artist "Doctor", advising a friend that with a $3000 DNA "scan", he can tell , based on the dna "results", which pharmaceutical drug anti-depressant is "best" for her daughter. I smell a fish. I hesitated to tell her she is being taken for a ride. The scam artist came recomended. Unsuspecting, well meaning, hard working classs people. trust in anyone's title of "doctor". Ive seen so many witch Doctors selling pills, powders, and potients to anyone ready to try anything. Its an epidemic. If Im wrong, just tell me, and I will have learned something. Thanks.

heard that engineered corn/soy now has been made so those plants/fruits produce their own "pesticides". Sounds like Star Trek. Can it be, people? Of course, this could start some histeria on us consuming these pesticides. Gotta be bunk. so far, could only find this: http://www.hoaxorfact.com/Health/gmo-corn-contains-harmful-insecticide-and-is-sold-unlabeled-facts-analysis.html

Ever since I first started masturbating I thought not producing pre-ejaculate was normal. Actualy I didn't know it was a thing until a few years ago. Now I realize I'm the odd man out and don't entirely know why. I think my bulbourethral gland is deficiant in some way, but I have not been able to prove it. Anybody with extincive knowlage about the male reproductive system is welcome and needed.