Biochemistry and Molecular Biology

I was wondering if any knows of any good papers that discuss target specific drug delivery vehicles for cancer. The articles can discuss their applications, method of development, sideffects, etc.... I am doing a 16 week coop with my chem prof. any help would be appreciated.

Hi there, I was just thinking as of why fungus grows on bread. What makes it to grow it ? Any help will be grateful...

Hi all, There are two questions which I'm having difficulty finding information on. Hoping someone can help me. I'm researching on the rationale for the addition of serum to tissue culture, I know serum consists of growth factors which may enhance cell growth but a serum-free cell culture is more desirable? Why? Secondly CO2 enriched environments: it acts as an natural buffering system, is there any other purpose to CO2 enriched environment? Maybe as a sterilisation technique as well? If anyone could help it would be highly appreciated:rolleyes: Thanks:D

Discuss protein specificity in terms of the genetic code? Can anyone answer this. PLEASE Thanks

Hey guys, I have a lab report due in 2 days and there are couple questions i just cant figure out. Its for a 200 level biochem class. The experiment has to do with acid percipitation of protiens. its just a very simple experiment where you percipitate cytochrome c and then milk protiens by the addition of 0.5% and then 20% acetic acid. one of the questions in the report is why does the cytochrome percipitate first when you add the acetic acid but then redissolve with the 2nd addition of the acid...does this have anything to do with the pH/ isoelectric point or am i way off? please if anybody could help me with this question i would really appreciate it.

hi, can anyone tell me what type of enzyme inhibition it is that reduces both Km and Vmax of a reaction? It's a question on a past paper for my med school exams. Any ideas?

Hi all, My friend is in his final year of graduation in Biochemistry. And he has absolutely no idea what to do next... He wants to have an idea of what can be taken up next, other than just plain biochemistry... Coz he feels, that Masters is a good time to specialise... What do u feel? Shud he continue with Biochem in his masters and specialise in his Ph.D? or should he specialise in masters itself? and wat are the possible fields he can venture into? and which is the best place in the world for a Doctoral and Post-Doc for this field? Kindly help... Prav.

The following segment of DNA codes for a protein (Uppercase letters represent exons, lower case letters represent introns): 5’GCTAAATGGCAaaattgccggatgacGCACATTGACTCGGaatcgaGGTCAGATGC3’ My question is this: What is missing from this sequence that we would need for the gene to make it's product if we were looking at an intact gene?

What would you expect of a chimeric molecule composed of N-terminal antibody fused with the 7TM portion of an GPCR? would the signaling go through if the antibody interacts with the antigen? Could you let me know if there is a forum for GPCR people? Thanks

Why is inoculating directly from glycerol stocks sometimes, but not always, a bad thing to do? (Instead of streaking out the stock first then inoculating from a single colony).

I dont know if I am in the right forum for this but I have a question. I am only 13 years old so please try to explain the answer in the easiest way possible =]]. Okay the other day we were talking in health class about how there will never be a cure for the common cold. I was thinking about this for days and days and I thought, " Is there a way that scientists could create a white blood cell that could transfor itself such as the cold or even aids and kill these diseases?" Can someone please tell me if there is a way or if I am just wondering dumb things.....?

Hello, I am a biomedical undergraduate student and I gotta write an essay on "Application of molecular techniques on histopathology". I'm not very familiar with molecular techniques because I didn't take any courses related to molecular biology. Can anyone of you give me some ideas about what molecular techniques are? and what kind of molecular techniques can be applied to histopathological analysis? I would be very thankful if some1 can give me some reference related to this topic. thankyou

Okay, so anyone have any experience with this? Lets say I have steptavidin coated magnetic beads coupled to 500pb Double stranded DNA via biotin. If I wanted to use this DNA as a template for PCR, I am presented with two ways of going about it. 1) Eluting the non-biotinylated strand of DNA, and using it as a template for PCR. 2) Using the beads with coupled DNA as template directly in the PCR reaction. This is preferable as there will be some DNA coupled to the beads that will not amplify and I can remove this contamination using the magnetic properties of the beads. So my question is which method is preferable. What kind of conditions would I need to elut…

Does anyone know which universities are best for studying biochemistry, in the States? Thanks!

Good day all, I'm a student and we are currently working on a project involving sterile filtration. The idea was to separate bacteria form a fermentor broth flow. Because it had to be a solution for filtering continous, I thought of a tangential crossflow filter. Luckily we had one at school, a labscale minitan TFF form the manufacturer millipore. I used the 0.45 mircometer pore membranes that where still in tact (4 membranes) and ran some tests on the fermentor broth. Fermentor broth contains: - LB medium - E coli (+enzyme) - p-phenylenediamine (*simulation) - 4-aminophenol (*simulation) - antifoam *The 2 simulation substances are there to simulate th…

In a recent research they found that although silent mutations dont change the amino acids in the polypeptide chain they can harm its function... so then there is something more between the protein and the DNA.... somethin that we definitely dont know...

Hello! I want to know, when counting the charge let's say of a simple peptide 5 amino acids let's say, i count only the residues of the positive lysine- arginine and histidine and negative- aspartate glutamate? Thank you.

What is the basic difference between the Rough Endoplasmic Reticulum and the Smooth Endoplasmic Reticulum?

Can anyone confirm this as being right? I'm reviewing for my photosynthesis test and these are among few that I have been dubious about. In the dark, or light independent reactions, carbon dioxide is fixed to ribulose biphosphate in the Calvin Cycle. The primary end product of this cycle is G3P, which may be converted to glucose and polymerized into starch. The molecule glycolate underoges subsequent metabolism such that it results in the release of a molecule of G3P. Light energy, captured by chlorophyll a, is transferred to a series of primary electron acceptors. The process whereby a plant uses a oxygen in light is photorespiration. Is green light t…

Hi I have a query about ordering DNA substrates for NMR...According to literature for a 1:1 ratio DNA:protein I need to titrate 10mM DNA substrate per NMR reaction. Most of the facilities that offer synthesis of oligos produce uM concentrations. Where can I order these quantites of DNA (in the UK), for NMR (I am also assuming that they need to be HPLC purified?)? Any help would be muchly appreciated

just a quick question... does anyone know the purpose of barbital buffer used in agarose gel electrophoresis? ? how does it affect protein movement? THX

I don't know if this is the right forum, but what are some good sources of vitamin B6?

Question: 1: what does the F in the general formula of glucose (CH20)n F stand for? 2:Why is it a 1-4c glycosidic linkage in amylopectin and amylose? why cant it be 1-5? 1-3?? 3: why do Polysaccharides lose their sweet taste? THANK YOU SO MUCH lol for whoever who has the answers!

I began dieting last week, but I feel as though if I were to be more educated in the area of biochemistry than perhaps I would know the proper foods to consume in order to achieve a great diet. My concerns are mostly of what calories, which I know doesn't necessarily fit under biochemistry, but my following questions will. Anyways, I understand that calories is food energy, which creates my state of confusion, for I'm under the impression that energy is good for us, which without it we would die. How by reducing food energy am I to lose weight? What would be considered a healthy level of calories? Now, to biochemistry. Obviously, I want to cut down own my carbohydrate…

anyone can shed some light on these puzzles as bellow, thanks. Microarrayanalysis has identified 3 previously uncharacterised genes that are strongly up-regulated in embryonic stem cells exposed to retinoic acid (embryonic morphogen). what assay can be applied to identify : 1. How many splice forms of each gene? 2. How big are the splice forms? 3. Where do each of the mRNA’s produced start and finish 4. Are they expressed in the developing embryo? 5. Where are they expressed? 6. Are they expressed in adult tissues and if so where? i think 1. northern blotting/ AgaroseGel Electrophoresis / 2. 3. In situ hybridisation, but which one would be better…