Biochemistry and Molecular Biology

Hey, I've found some ways to extract proteins from diatoms and I'm going to start trying them. They aren't the easiest to work with but they're fascinating. I was wondering if anyone here has worked with them and knows which one is best. Or even if you have any tips. Greetings

What is the essential difference between two strains of E.coli: BL21(DE3) and TOP10? Why is BL21 used for protein expression and TOP10 for plasmid storage? The plasmid in this case is pDEST17. I'm really new at all this and looking at genome of these two doesn't give me any answers... I know it has something to to with lack of lac operon in TOP10 strain. Please help.

Hi! I am working on a project on types of RNA. I heard that RNA subunit of telomerase is small nuclear RNA. Then what is the difference between small nuclear RNA and telomerase RNA?? and also the repeats that telomerase adds on, what is the termination codon in that case?? How does it stop from adding on the repeats? Can anyone please help me with this??

i was wondering about the mechanisme of chemical synapses if there anyone to explain it with details .

In yeast two hybrid (Y2H) system, binding domain (BD) and activation domain (AD) of GAL4 protein are separated and linked to heterologous protein. Besides the protein structure of BD-AD, I have also heard of BD-repressor domain structure. The problem is I don't know of any example, could anyone suggest some?

Adrenaline is designed to give you a temporary boost in energy and power, in order to overcome danger. Well, for each milligram of adrenaline that your body produces, how many joules of energy does your body get? Feel free to group it into kilojoules or megajoules if you like.

I am trying to figure out how to measure the corticosterone release by cells in culture when stimulated by agonists, and I need help figuring out how a liquid scintillator measures this. Please help. Thanks

The topic title pretty much states my purpose. I'm trying to gain an idea of the effects on total protein production the knockdown of a specific gene product has on Drosophila larvae. My professor would prefer if I could find a method that does not rely on radio-labeled amino acid incorporation so that I can perform the experiments without his supervision. However, I'm having trouble finding any such methods. Does anyone know of any labeling methods that might work for what I'm trying to do? As I noted in the topic, we're going to be measuring in vivo, so any in vitro methods won't work, and I don't think fluorescent antibody labeling is feasible either, as we're trying t…

Hi, As part of my degree I am studying PCR analysis of DNA. I will be studying the effects of different enzymes have on the DNA after. So for example, in people who are lactose intolerant, I will study the gene sequence before and after the addition of B-Galactosinase. I am wondering if anyone knows of a good databse with the pathways for the genes/enzymes of interest. A good example is this one I found myself; http://www.galactosaemia.com/galactosaemia/metabolism.html I want to look into a number of disorders too so any advice or where to look for the genetics of the diseases and their metabolic pathways is greatly appreciated! Thank you!

Treatmentof a tetranucleotide with Snake Venom Phosphodiesterase yielded pGp, pT, pC,and pC. Prior treatment with aphosphomonoesterase (phosphatase) followed by Snake Venom Phosphodiesteraseyielded pC, pC, pG, and T. What is the sequence of the tetranucleotide? Explainyour reasoning. If someone could put me in the right direction

Hey everybody. I'm a final year student, and I have to do some experiments with SSR (simple sequence repeat) indicators. Now I'm preparing to cloning some genes, and I have some troubles. It's the DNA extraction stage, but from 6% silver- staining polyacrylamide gel (normally, in my lab, they just extract from agarose gel). I need some advices about the protocol and the difference between the ethidium bromide - staining polyacrylamide gel and the silver - staining one which I have to be noticed before I go to extract DNA. The interested band is about 300 bp and I have to electrophoresis PCR products on polyacrylamide gel because there're many bands which have their size …

As asked in the title ?

i was wondering about atp how could this molecule be the key of bioenergetics and how the atp is builded by the sphere pedenculate (in the cell membrane)

Hi all, Is there an extracellular reaction that can convert electric energy into chemical energy stored in ATP ? I know the reverse process occurs in electric eels. I am trying to develop a biomimetic electric device. Thank you, R

For complex living organisms and DNA virus, genetic information comes from DNA. For RNA virus genetic information comes from RNA. Are complex living organisms genetically more closely related to DNA virus than RNA virus ?

I'm thinking of applying for a degree in Medical Biochemistry in september, I'm really into Biology and love the subject, the areas which have interested me most have been those on a molecular level so I thought biochemistry would be perfect. The only thing I'm worried about is the level of Chemistry, I'm really not into chemistry that much. When i look at the specification for the course it seems to be all Biology related, I'd just like to know how the Chemistry fits in and if im making a right decision? If theres any on here whos done a degree in biochemistry or knows alot about the subject could they please help? thanks

hi...i'm working on the enzymatic hydrolysis of lignocellulosic biomass. I have been reading literatures about effects of enzyme loading on sugar yield. I'm very confused about the units they use, FPU, CBU, IU, etc. since this is not really our focus in Chemical Engineering. I need to add 30, 50 and 60 FPU of enzyme per g cellulose. But my literature gives me the enzyme activity as 3200 IU/g. can somebody please help me know how to convert this? Or is there really a way to do it? Should I do the filter paper assay procedure? Please please...need your help.

So life is made from chemicals with specific handedness. But as far as I know, it should in theory work just as well with all the handedness reversed. I'm not sure if we could actually make one though, due to technological difficulties. If we could make life with the opposite handedness, how would you go about making it? And a related question, could we eat the result?

I'm having trouble to clone some genes on DH5alpha E. coli strain. When I sequence the insert, I can only get 600 bp instead of the original size of the insert (1,2 kb). Does anyone know if this strain has restriction enzymes that can cut the insert?

What is a broth in microbiology?

how cell lines are made?

The question is regarding the electron transport chain within the mitochondria, specifically how the electrons transport protons through the first and third complex. The basis of this question can be found at the tutorial at ... http://www.johnkyrk.com/mitochondrion.swf As each pair of electrons passes through each individual complex, they cause the transport of two protons across that complex from the matrix to the outer chamber. The electrons move by passing from the NADH/FADH2, to the flavin inside the complex (FMN) and then “bouncing” between the iron cores of Heme molecules (porphyrin rings) also within the complex. In the process of jumping betwe…

Actually I am looking for plasmids containing MV-H and MV-F, I surf companies' website but could not find such a product. I need them because I am planning an experiment related to measles H and F mediated cell fusion, all other materials are ready except this pair of plasmids. So is there any suggestion where I can find them. Or if there is any scientists here, could they kindly provide some for me? Thanks.

i am not on the computer much and i know science like i know chinese(which i currently do not) that said, ... there are micro organisms that you "grow" and maintain in sand, that, combined with other filters like charcoal,etc. filters water to drinkability...? do i need specific type of sand...better question could anyone suggest some super good somewhat easy to understand reading materials?

I have a quick Spectrophotometry question! A solution of the nucleotide base uracil, in a 1mm cuvette, has an absorbance at 260 nm of 0.65. Pure solvent in a matched quartz cuvette has an absorbance of 0.07. What is the molar concentration of the uracil solution? Assume the molar absorption coefficient is 8.2 x 103 M-1 cm-1