newbie17425 Posted June 5, 2015 Share Posted June 5, 2015 Hello, I want to understand how do i analyze melt curve after my qPCR? What are the different curves? I am just aware that melt curve analysis is done to check for secondary products or primer dimer formation. I would appreciate your help in understanding the melt curve. Thanks Link to comment Share on other sites More sharing options...
CharonY Posted June 5, 2015 Share Posted June 5, 2015 What curves the systems gives you is dependent on the system. Typically it is a simple fluorescence (usually normalized) over temp plot with a curve per sample. However, I wonder, if you are supposed to do this experiments is the lab/course not explaining you how the systems work? Link to comment Share on other sites More sharing options...
newbie17425 Posted June 6, 2015 Author Share Posted June 6, 2015 For some reason i cannot upload a picture here. I just wanted to know what does the peak represents? And if i had secondary products/contamination/primer dimers then i would have extra peaks right? Link to comment Share on other sites More sharing options...
StringJunky Posted June 6, 2015 Share Posted June 6, 2015 (edited) For some reason i cannot upload a picture here. Click "More Reply Options". bottom right of message input box. Perform in order as shown: Edited June 6, 2015 by StringJunky 1 Link to comment Share on other sites More sharing options...
newbie17425 Posted June 6, 2015 Author Share Posted June 6, 2015 Thanks for guiding me on how to upload the pictures. Here are the pictures The first picture is of the NTC and the other two are of the 2 unknown samples in triplicates. Link to comment Share on other sites More sharing options...
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