Hi all,

can you tell me how I prepare a stock solution of 10% SDS? What does 10% mean in this case? 10mg in 100 ml?10 g in 100 ml ?

Thank you very much.

Silvia

Do you have protocol? If so concentrations should always be indicated by weight and/or volume (e.g. w/w, v/w, v/v). Of course in case of solids weight is usually used.

I have a protocol but it says just to add 40 ul of 10% SDS . The SDS I have is in powder.. So, I can suppose that I have to weight 10 g and dissolve them in 100 ml, haven't I?

SDS is a solid, so a 10% solution will be 10% weight of SDS in water, 10g per 100ml

The weights and volumes aren't critical.

 

Are you a student or working in a lab?

What background do you have?

SDS is a solid, so a 10% solution will be 10% weight of SDS in water, 10g per 100ml

The weights and volumes aren't critical.

 

Are you a student or working in a lab?

What background do you have?

 

What do you mean by the weight and volumes are not critical? Or do you mean that the difference for water between weight and volume percentages is not critical?

Just that 10% SDS need not be made up volumetrically. You are not going to get much difference in action between 9% and 11%, I used to make it up 10g +-0.2g in 100ml water.

That depends a lot on your application. I doubt that it is the case for OP but if you need quantitative reproducibility or are investigating high-resolution interactions with surfactants you better be more precise. If you want to work at the critical micelle concentration, for example being even 1mmol off can be problematic, depending on your analysis.