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Optical density = concentration

Gamewizard
in Homework Help

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Hi quick question

 

What would be the bacterial concentration at 0.6 OD ? and how would you write that as ?

 

thank you

Hi, OD=0.6 only means that the power of light passing through is divided by 100.6. You would need the light's path length and some attenuation (possibly by diffusion) properties of these specific bacteria to deduce a concentration, provided nothing else attenuates.

That is absolutely dependent on your bacterium and the medium you are using. You will either have to count the cells or plate them (life titer) for a given OD range and plot a calibration curve.

  • Author

Thank you for your replies, I will have to do plate counts for it

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