An 8000 bp plasmid construct has 2 restriction sites for EcoRI at 0 bp and at 5000 bp as well as a restriction site for HaeIII at 1000 bp. If the plasmid was digested with HaeIII, how many fragments would be generated, and what are their sizes?

if it was cut at 1000bp by HaeIII then wouldn't it be just one fragment?

but i am confused as to why they would also have EcoRI in there?

so the answer to this question would 1 fragment with a size 6000bp? right?

Also posted in homework.

!

Moderator Note

I have closed the other two identical threads. AsianEinstein, please do not open multiple threads on the one topic.

sorry new to the forum I will keep that in mind for future thank you

It seems no one can help you here on scienceforums. Hope you can find help elsewhere.