hey_howudoin2003 Posted November 10, 2011 Share Posted November 10, 2011 Is it possible to ligate a vector cut by 2 different blunt ended restriction enzymes? Link to comment Share on other sites More sharing options...
CharonY Posted November 10, 2011 Share Posted November 10, 2011 Why shouldn't it be possible? Link to comment Share on other sites More sharing options...
hey_howudoin2003 Posted November 10, 2011 Author Share Posted November 10, 2011 Will it be of good efficiency???? Link to comment Share on other sites More sharing options...
CharonY Posted November 10, 2011 Share Posted November 10, 2011 It will be of lower efficiency than sticky end. Link to comment Share on other sites More sharing options...
hey_howudoin2003 Posted November 10, 2011 Author Share Posted November 10, 2011 So, I already have a promoter cloned in a vector and I intend to study expression patterns by doing promoter bashing where I digest the DNA with blunt ended enzymes and removing the small piece and re ligating the remaining fragment. Link to comment Share on other sites More sharing options...
shaung Posted December 5, 2011 Share Posted December 5, 2011 ligation shouldnt be an issue if your are just excising a fragment, as long as you do the obvious, i.e. gel purification of the vector, otherwise you could just ligate the excised fragment back into it's original position. Link to comment Share on other sites More sharing options...
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