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hey_howudoin2003

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3
Joined
November 10, 2011
Last visited
November 10, 2011

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Biology

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Blunt ended ligations

hey_howudoin2003 replied to hey_howudoin2003's topic in Biochemistry and Molecular Biology

So, I already have a promoter cloned in a vector and I intend to study expression patterns by doing promoter bashing where I digest the DNA with blunt ended enzymes and removing the small piece and re ligating the remaining fragment.
- November 10, 2011
- 5 replies
Blunt ended ligations

hey_howudoin2003 replied to hey_howudoin2003's topic in Biochemistry and Molecular Biology

Will it be of good efficiency????
- November 10, 2011
- 5 replies
Blunt ended ligations

hey_howudoin2003 posted a topic in Biochemistry and Molecular Biology

Is it possible to ligate a vector cut by 2 different blunt ended restriction enzymes?
- November 10, 2011
- 5 replies

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