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Origin of Replication Compatability

apricimo
in Biochemistry and Molecular Biology

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Looking to coexpress two vectors pCDFDuet and pETDuet in E. coli. Origins of replication are CDF and F1 respectively. Anyone know if these are compatible?

 

Yes they are compatible

IIRC the duet vectors are routinely used for multiple co-expression, so as long as you use a host strain that is compatible to both plasmids, you shold be fine. Novagen also sold at least one of those. Novablue or something like that. Other strains include B834 and/or Bl21 derivatives, I think. But better check that out, as my recollection is a bit hazy on that.

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