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Sau3A enzyme: What is it cutting?

Genecks
in Genetics

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I'm looking at the action of the Sau3A enzyme.

What exactly is it cutting?

 

5'---

 

What does that third hyphen represent?

 

Picture attached.

Sau3A.jpg

It doesn't matter what the third hyphon it is. I ASSUME it could be any base and that the sequence it needs for recognition is just GATC. However, I'll just check that and get back to you in a second.

 

 

Just looked it up on NEB cutter and that's correct.

Edited by Greippi

5'---GATC---

3'---CTAG---

 

it gets cut as shown. The hyphens are just placeholders.

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