Jump to content

SDS agarose gel electrophoresis

Featured Replies

I know that:

bromophenol blue – used as a marker since most proteins and nucleic acid are colorless.

glycerol - It is a preservative and a weighing agent.

 

but what's the purpose of SDS and tris base in SDS agarose gel electrophoresis

It depends on what kind of electrophoresis you are doing. For proteins generally polyacrylamide gels are used instead of agarose. The reason why SDS is used is easily answered. In order for gel electrophoresis to work all the particles should have roughly similar charges (at least no inveresd polarity). Think about proteins, is that the case? For some other specialized applications SDS can have other uses including cell lysis in-gel.

Archived

This topic is now archived and is closed to further replies.

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.

Configure browser push notifications

Chrome (Android)
  1. Tap the lock icon next to the address bar.
  2. Tap Permissions → Notifications.
  3. Adjust your preference.
Chrome (Desktop)
  1. Click the padlock icon in the address bar.
  2. Select Site settings.
  3. Find Notifications and adjust your preference.