Jump to content

Why do smaller particles travel faster in gel electrophoresis?

Featured Replies

Hello guys,

 

In electrophoresis I mean sorry.

 

In electrophoresis is a similar electromotive force applied to all particles or are all particles accelerated at the same rate?

 

What I mean is let's say if every particle gets 10N.

 

F=ma and bigger particle would travel slower. So can't I include this in the answer. If they are only accelerated at the same rate I can understand how different particles would have different resistances?

 

So is the answer F=ma?

 

Thank you!! :)

Edited by scilearner

It is slightly more complicated than that. Generally the major forces in such systems are electrophoresis and electroosmosis (the latter mostly in capillary or smaller system and assuming a homogeneous field). The electrophoretic component is determined by the electrophoretic mobility of the particle in the given system.

The force of acting on the particle scales with the charge, although geometry also has an effect (as well as media properties). Generally, however, the size effects tend to be negligible compared to the charge (on which the field really acts on).

The size dependent resolution in some standard electrophoresis techniques is based on the use of additional tricks (i.e. gels) to achieve separation in cases where the electrophoretic properties of the particles alone are insufficient or not well-defined (e.g. in case of biomolecules).

Archived

This topic is now archived and is closed to further replies.

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.

Configure browser push notifications

Chrome (Android)
  1. Tap the lock icon next to the address bar.
  2. Tap Permissions → Notifications.
  3. Adjust your preference.
Chrome (Desktop)
  1. Click the padlock icon in the address bar.
  2. Select Site settings.
  3. Find Notifications and adjust your preference.