Sohzi Posted January 24, 2010 Share Posted January 24, 2010 Plaque assay is performed beggining with a 10 mL phage solution. The solution is serially diluted 4 times by taking 1 mL and adding it to 9 mL. 0.1 mL of the final dilution is plated & yields 21 plaques. What is the initial density of the starting phage solution? So here's what I know. Density= (# of colonies formed/ mL plated) x Dilutionbefore plating # of colonies formed is 21. Now I'm assuming the mL plated is 0.1 mL? And as for the the dilution I am stuck. Is there a way I can figure out how to calculate that? Link to comment Share on other sites More sharing options...
CharonY Posted January 24, 2010 Share Posted January 24, 2010 You take 1 ml and fill it up to 10 ml. What amount of dilution is that? Link to comment Share on other sites More sharing options...
Sohzi Posted January 24, 2010 Author Share Posted January 24, 2010 So if we do that four times it becomes 10000 more dilute. Is that my dilution number I'm looking for? Link to comment Share on other sites More sharing options...
CharonY Posted January 24, 2010 Share Posted January 24, 2010 Yupps. Link to comment Share on other sites More sharing options...
Sohzi Posted January 24, 2010 Author Share Posted January 24, 2010 Wow awesome. So... (21 plaques/ 0.1 mL plated) x 10^5 = 2.1x10^7 PFU/mL So I did it perfect? 100%? Link to comment Share on other sites More sharing options...
T4phage Posted January 24, 2010 Share Posted January 24, 2010 yeah, that's what I got when I did it on the assignment Link to comment Share on other sites More sharing options...
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