Plaque assay is performed beggining with a 10 mL phage solution. The solution is serially diluted 4 times by taking 1 mL and adding it to 9 mL. 0.1 mL of the final dilution is plated & yields 21 plaques. What is the initial density of the starting phage solution?
So here's what I know. Density= (# of colonies formed/ mL plated) x Dilutionbefore plating
# of colonies formed is 21.
Now I'm assuming the mL plated is 0.1 mL?
And as for the the dilution I am stuck. Is there a way I can figure out how to calculate that?
