glo1000 Posted January 27, 2009 Share Posted January 27, 2009 First off, I am sorry for being blunt with my questions but I am honestly stumped. Can anyone lend a thought? The first question I have no idea how to even get started. Estimate the copy number of pET22b+ in the average E.coli cell. You may assume that there are approximately 1 X 109 E.coli cells in each mL of overnight-saturated culture. The size of the pET22b+ plasmid is ~5500 bases, the size of the insert is ~500-800 bases, and the average molecular weight of a nucleotide base pair is 660 g/mol. Or this question: How could you use this sequence to design a 5’ primer that could sequence your own DNA to determine if you have any genetic polymorphisms in your MIC or NKG2D genes? Design such a primer. Link to comment Share on other sites More sharing options...
CharonY Posted January 30, 2009 Share Posted January 30, 2009 What is the weight of one plasmid? Link to comment Share on other sites More sharing options...
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now