glo1000

First off, I am sorry for being blunt with my questions but I am honestly stumped. Can anyone lend a thought? The first question I have no idea how to even get started. Estimate the copy number of pET22b+ in the average E.coli cell. You may assume that there are approximately 1 X 109 E.coli cells in each mL of overnight-saturated culture. The size of the pET22b+ plasmid is ~5500 bases, the size of the insert is ~500-800 bases, and the average molecular weight of a nucleotide base pair is 660 g/mol. Or this question: How could you use this sequence to design a 5’ primer that could sequence your own DNA to determine if you have any genetic polymorphisms in your MIC or NKG2D genes? Design such a primer.