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uncut DNA in electrophoresis!! help!!

mk_2007
in Biochemistry and Molecular Biology

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Hi! I was wonderng if anyone knows why its useful to include uncut DNA in an electrophoresis when analysing the cut DNA restriction fragments?

As otherwise you won't know if the Cut DNA has actually been cut up or is just the normal DNA, you can work it out of course with markers but it is easier just to have two bands form.

  • 3 weeks later...
Hi! I was wonderng if anyone knows why its useful to include uncut DNA in an electrophoresis when analysing the cut DNA restriction fragments?

 

seriously, people, don't answer questions when it's for homework/school assignments!!

 

Ready your book kid!

  • 1 month later...

That's a perfectly legitimate question that I myself asked while working in the lab.

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