ecoli Posted March 20, 2008 Share Posted March 20, 2008 Does anyone know how the leucine-responsive regulatory protein works to regulate gene expression? From what I understand, in the pap operon in E. coli, it binds to a operator overlapping a Dam site. By blocking Dam, it prevents the expression of the papI promoter. I don't understand how the protein functions, however. The paper I'm reading doesn't really go into detail about it. Link to comment Share on other sites More sharing options...
CharonY Posted March 21, 2008 Share Posted March 21, 2008 I am not sure what your question is. Proteins of the Lrp-family bind DNA via a N-terminal HTH motif (pretty much standard in that regard). Generally, if you find that a particular paper is missing information that you need, try to track down a review. There are a couple around of LRP-type proteins. If you are interested what the Lrp-protein in E. coli does, there are number around describing its regulon (it is rather a global regulator IIRC). Or are you actually interested in the particular mode of the Lrp-regulated Pap-pili switch? That is, how methylation is inhibited? Link to comment Share on other sites More sharing options...
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