In the past I followed a protocol based on Methods in Enzymology Volume 174. We warmed the tubing a bicarbonate/EDTA buffer to about 80 °C. We rinsed several times with warm DI water. We stored in the presence of a preservative, often sodium azide. Is this similar to what other labs do?

EDT

Does anyone have experience with SnakeSkin dialysis tubing? It seems to be much less expensive than the alternative, although that may be because of a promotion.

Edited June 20Jun 20 by BabcockHall

On 6/20/2025 at 7:33 AM, BabcockHall said:

In the past I followed a protocol based on Methods in Enzymology Volume 174. We warmed the tubing a bicarbonate/EDTA buffer to about 80 °C. We rinsed several times with warm DI water. We stored in the presence of a preservative, often sodium azide. Is this similar to what other labs do?

Pretty much. Though we do not add a preservative, we generally use sterilized solutions and keep it that way if we are using them. I believe in rare cases (and with compatible material) we also had stored them in ethanol, but rarely.