ssarenkaa Posted January 14, 2021 Share Posted January 14, 2021 hi, how can i optimize this gel for better electrophoresis? Link to comment Share on other sites More sharing options...
CharonY Posted January 15, 2021 Share Posted January 15, 2021 Fundamentally I do not think that there is a much wrong with your gel (for most purposes that is). The left pocket might have been damaged a bit. You have a bit of a frowning effect going but nothing too worrisome (though I would add ladder at the last lane to address that a bit. Link to comment Share on other sites More sharing options...
Arete Posted January 15, 2021 Share Posted January 15, 2021 Apart from the points CharonY made, I don't see a negative control, or if there is one you have a contamination issue. Are you actually asking how to optimise the PCR given the multiple banding? I would say use a touchdown protocol, or optimise your primers, but I would probably cut out my gel bands and call it a day. Link to comment Share on other sites More sharing options...
CharonY Posted January 15, 2021 Share Posted January 15, 2021 58 minutes ago, Arete said: Apart from the points CharonY made, I don't see a negative control, or if there is one you have a contamination issue. Are you actually asking how to optimise the PCR given the multiple banding? I would say use a touchdown protocol, or optimise your primers, but I would probably cut out my gel bands and call it a day. Well that is always assuming that one expects a singular target. As far as I can tell it is unclear what kind of assay it was in the first place. Link to comment Share on other sites More sharing options...
ssarenkaa Posted January 15, 2021 Author Share Posted January 15, 2021 thank you for answers Link to comment Share on other sites More sharing options...
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