I have a question about HPLC. My peak is very small (it’s more like a little line). It is definitely not like Gauss peak, but I have to find column efficiency. Can I calculate it using the formula Ne=16*(t’r/Wb)^2? Or what else should I do? I found the information that I can only calculate it when the peak is big.

Edited May 9, 20205 yr by txxplc110

30 minutes ago, txxplc110 said:

I have a question about HPLC. My peak is very small (it’s more like a little line). It is definitely not like Gauss peak, but I have to find column efficiency. Can I calculate it using the formula Ne=16*(t’r/Wb)^2? Or what else should I do? I found the information that I can only calculate it when the peak is big.

Not enough information.

Did you for instance have the flushing speed wrong (too fast)  ?

20 minutes ago, studiot said:

Not enough information.

Did you for instance have the flushing speed wrong (too fast)  ?

These are not my results and I don't have any other informations. I'm only analyzing it.  I can't publish the exact chromatogram, but it looks like the one on the picture. I am worried about the high value.