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txxplc110

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About txxplc110

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    Lepton
  1. Hi! Which of these points would be a dead time? 2.3 is not marked with a number so is it 3.7?
  2. These are not my results and I don't have any other informations. I'm only analyzing it. I can't publish the exact chromatogram, but it looks like the one on the picture. I am worried about the high value.
  3. I have a question about HPLC. My peak is very small (it’s more like a little line). It is definitely not like Gauss peak, but I have to find column efficiency. Can I calculate it using the formula Ne=16*(t’r/Wb)^2? Or what else should I do? I found the information that I can only calculate it when the peak is big.
  4. Hello! I have a question about HPLC chromatograms. I got two chromatograms, each one with different signal/wavelength (290nm and 325nm) and I got two peaks of compounds that I wanted to analyze, but the peaks are not on the same chromatogram. My question is, can I read peak resolution of two peaks from two different chromatograms, or not? It doesn’t make any sense for me, but it's in my instruction. I can’t find any information about that. I would be most grateful if you would look into this matter.
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