BabcockHall Posted March 7, 2020 Share Posted March 7, 2020 link Good Morning Everyone, I have been studying various forensic test kits for body fluids, and a number of them from Abacus Diagnostics or Independent Forensics use lateral diffusion immunochromatography. In a sandwich format the antigen forms a complex with both the soluble, labeled antibody and the antibody immobilized onto a membrane at the test (T) position. I can see how this could easily work with a protein such as hemoglobin, which is a tetramer of two alpha and two beta subunits. It is less clear how this assay works with human salivary alpha-amylase, which is a monomer from what I can gather. In other words I can see how the sandwich forms when there are multiple epitopes, but it is less clear to me what is happening when there might be only one epitope. Link to comment Share on other sites More sharing options...
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