Hello

I have prepared Bradford's reagent using the various protocols found online and also mixed some protocols to get the desired color.

I am facing a problem that i want to know the quality of the reagent is right or wrong i want to know whether there are  any test ? or some methods to actually check the reagent made in the lab.

Kindly let me know

Thank you in advance. 

I am not aware of any paper that specifically addresses this question.  There are experiments one could do.  For example, it has been reported in the product literature from BioRad that bovine serum albumin gives about twice the response as egg albumin when both are at the same concentration in mg/mL.

I'd do some standard tests for dynamic range, detection limit, reproducibility, etc. I.e. create repeat calibration curves with standard protein of known concentration. You can also compare them with commercial reagents, if you have them.