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Monomer

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Everything posted by Monomer

  1. The internet!! Go a google search on RT-PCR, and check out google scholar and pubmed to find out what others have done. That's what I do when I'm starting on a new technique that I'm unfamiliar with. Certainly take the initiative and gather as much information on your own, but if you're a beginner then don't feel bad about asking your professor questions.
  2. This site states that Also found this: http://www.abc.net.au/health/thepulse/stories/2005/09/15/1460760.htm More info: http://www.kidney.org.au/KidneyDisease/Drinkwaterinstead/tabid/703/Default.aspx So, know your body and give it what it needs, bearing in mind that the body's needs will change with changing circumstances.
  3. Have you asked your supervisor why there might be a colour difference? I make up cell culture media and a difference in colour is an indication of a pH problem.
  4. I tend to make lots of silly mistakes when I'm stressed, so you definitely need to learn to relax. If you want to get through your PhD and work in research you won't last too long and you won't get a good reference if you keep making silly mistakes. I know the pressure is on for you this year, but take plenty of breaks, get lots of exercise, eat well, and make time for the things you enjoy. Even though you have a difficult project to get through this will be a very valuable year and the experience will be incredibly beneficial. You don't have much lab experience, but everyone starts at that point. It's a steep learning curve but hopefully you'll find it quite rewarding.
  5. Another thought on the popping sound... my ankle pops alot and the physio explained that it occurred when the ligaments moved against the joint. It could be a similar thing in your knee and if it's happened more since you've started going to the gym then maybe it's because you're stretching those ligaments more...? Anyway, like iNow said, let us know the doctor's opinion.
  6. A chiropractor I saw told me the popping sound was air escaping from the joints. I assumed the guy knew what he was talking about, but I just checked it out and it appears to be the reason. Apparently if there's no pain with the noise then it's okay, but if you experience pain then there's a more serious issue. Getting into better shape and strengthing the muscles is recommended, and I also think it's better to avoid exercises that put too much stain on the knees, like running, so hopefully the elliptical machine will benefit you. http://sportsmedicine.about.com/cs/injuries/a/aa092500.htm http://orthopedics.about.com/cs/arthritis/g/crackingjoints.htm http://www.orthop.washington.edu/uw/arthritisbasics/tabID__3376/ItemID__180/PageID__348/Articles/Default.aspx
  7. Were the pin holes all over the gown or were they concentrated in a particular area? I think it's important to know how surgicaltech got it. If she continues to work as a veterinary technician with the knowledge that the x-rays (maybe) caused the cancer then she can avoid doing x-rays rather than risk cancer again.
  8. You can just make up your own acronym - it might be easier for you to remember.
  9. This is still being debated. Some say it's bad, some say it's good. http://mail.turkiye-klinikleri.com/cgi/content/abstract/14/11/1081 http://jama.ama-assn.org/cgi/content/abstract/287/16/2081 http://www.ajph.org/cgi/content/abstract/84/8/1319 And there's the question of whether milk increases mucus production. Some people have an intolerance or allergy to milk, but for others a bit of dairy a day doesn't seem to be too harmful. Again, there are some that say raw milk is the best because pasteurisation destroys the vitamins in the milk, but pasteurisation also destroys the nasty bacteria. I think that if your body isn't showing any adverse affects when you consume milk then you can continue to drink it. Don't over do it though; it would probably be best to stick to the recommended daily calcium intake levels. And stick to low fat milk. But also remember that calcium can be found in other foods, and it's important to have a balanced diet. http://www.betterhealth.vic.gov.au/bhcv2/bhcarticles.nsf/pages/Milk_the_facts_and_fallacies?open Something else to read: http://www.guardian.co.uk/lifeandstyle/2003/dec/13/foodanddrink.weekend
  10. The scientific american article and another article I found, basically state that scratching provides a painful distraction, taking the mind off the itch. http://query.nytimes.com/gst/fullpage.html?res=9E00E3D6143AF932A35754C0A9659C8B63&sec=health&spon=&pagewanted=2 http://www.sciam.com/article.cfm?id=chronic-itching So the scalding water is having the same effect as scratching because it's activated the pain C fibres, and maybe it's having the best, longest lasting effect because it's causing more pain, and perhaps more damage...
  11. It's interesting because as the study states "teens who are active in religious services are depressed less often because it provides these adolescents with social support and a sense of belonging". Religion fills people with a sense of hope and joy; there's a higher calling and all that happens isn't random but it's for a particular purpose. However, I can see that for a teenager who is trying to understand themselves and find their place in the world it would be a struggle to negotiate the demands of religion and society. Depression was higher among minorities highly involved in church. As a minority they are already struggling to fit in, but their acceptance of religious beliefs and principles, which contrast those of society, set them apart even further. Those that were depressed probably had lower self-esteem because I would think that those with confidence would be better able to decide what they believe and have the ability to stand firm despite surrounding pressures. Perhaps those depressed individuals also feel that they don't fit the religious "ideal" and may think that it is too far beyond their grasp. It might also be that those who were depressed feel guilty for living as the "world" lives with the knowledge it goes against their religious beliefs.
  12. What about these sites, or are you looking for a more detailed explanation? http://www.rpi.edu/dept/bcbp/molbiochem/MBWeb/mb1/part2/glycolysis.htm http://en.wikipedia.org/wiki/Glycolysis http://themedicalbiochemistrypage.org/glycolysis.html http://mcdb.colorado.edu/courses/3280/chime/glyco/index.htm
  13. To find info on the internet you need to be persistant. Try different search phrases and words, and look well beyond the first page of results. Here's what I found: Vitamin D: http://jn.nutrition.org/cgi/content/full/135/11/2739S http://vitamind.ucr.edu/milk.html Erythromycin: There seems to be some info in this book, but they don't show all the information. Maybe you could see if it's available in a library near you.
  14. http://www.nsc.gov.sg/content/613/NSC%20Bulletin%20Vol_17_2.pdf#page=15 http://en.wikipedia.org/wiki/Gate_control_theory_of_pain I've also read that hot water draws out the histamine that's causing the itchiness, so you could look into that. As for why scratching brings relief, take a look at these: http://www.newsweek.com/id/106588 http://www.sciencedaily.com/releases/2008/01/080131121804.htm
  15. Maybe these will answer your question: http://www.scienceblog.com/community/older/1999/E/199904719.html http://www.sharpbrains.com/blog/2008/02/05/stress-and-neural-wreckage-part-of-the-brain-plasticity-puzzle/ Something else to read: http://www.sfn.org/index.cfm?pagename=brainBriefings_stressAndTheBrain
  16. I've heard of industrial funded PhD scholarships in Australia.
  17. Am I too late? It seems that HIV and HSV can "hide" in the CSF. Check out this paper: http:// http://en.wikipedia.org/wiki/Blood-brain_barrier http:// http://www.encephalitis.info/TheIllness/TypesEncephalitis/HSE.html Yes, but it seems to be only in the case of inflammation. Here's some info I found: http://www.medicalnewstoday.com/articles/42306.php Have a read of this: http:// http://books.google.com/books?id=NWBtIpoJ5m4C&pg=PA114&vq=inflammation&dq=t+cells+enter+cns&source=gbs_search_s&sig=ACfU3U2dTKfdJzugNR2f9kvPUMJNZUCjQA#PPA114,M1 Maybe you'll find this interesting aswell: http://www.geocities.com/HotSprings/3468/immucells1.html
  18. I'm not a doctor, so I don't know for sure, but it seems that "trilineage" just confirms that 3 cell lineages are affected. There's no "bilineage" pancytopenia, but there's bilineage cytopenia. Trilineage cytopenia is pancytopenia, so maybe at sometime "trilineage" was added to pancytopenia for confirmation and it's stuck, but it is really redundant.
  19. From what I remember when studying biochemistry, physics would be least essential. Physical Chemistry and Quantitative Analysis would be far more useful to you.
  20. According to this site, "Other causes include any process filling the air spaces with blood, fluid, or inflammatory cells. Alveolar collapse (atelectasis) also causes consolidation." This book mentions TB as a cause of pulmonary consolidation, along with systemic lupus erythematosus, carcinoma, pulmonary infarction, immunological disorders (sarcoidosis), adult respiratory distress syndrome. Check out this book too.
  21. Hi everyone! I've been looking around here for a while and finally decided to join. I'm a uni science graduate and I currently work as a lab technician. I dabble in a bit of microbiology, molecular biology, genotoxicity, cell culture, analytical chemistry and whatever else my boss throws at me! I'm here to learn and to hopefully impart some of my wisdom... now where did I put it... See you around!
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