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donde

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Everything posted by donde

  1. i have sent the abstract. thank you very much for your help!
  2. Thank you for your help! I' d be interested on your comments on the content too
  3. Hi everyone, I am an italian biologist. I'm glad to be here
  4. hi, where are you from?
  5. Hi to all, I'm an italian biologist and I had to translate the abtract of my two thesis. Before sending them, I want to be sure that they are correct. Please take a look and kindly tell me if there is any error. Please report grammar, sintexical or phrase construction errors. If you want, you can also give me suggestions about how to write them. Thank you very much. Here they are: Abstract 1: Connexin43 gap junctions are necessary for skeletal muscle repair, which requires the proliferation and differentiation of satellite stem cells. The sphingolipid sphingosine-1-phosphate can bind to specific receptors. Its synthesis depends on the hydrolysis of sphingomyelin by sphingomyelinase and on the action of sphingosine kinase. The aim of this study was to assess if the sphingosine-1-phosphate-mediated regulation of differentiation and proliferation of myoblasts depends on the expression of connexin43 and on the formation of gap junctions. The results show that the phosphorylation of sphingosine and the transport of sphingosine-1-phosphate outside of the cell are necessary for the increase of connexin43 expression during early differentiation of C2C12 myoblasts, which is suppressed by the inhibition of sphingosine kinase or the sphingosine-1-phosphate transporter. The activation of S1P1 and S1P3 isoforms of sphingosine-1-phosphate receptor is important, as demonstrated using agonists, antagonists and siRNA specific for the receptors. Primary culture myoblasts derived from Cx43 knockout mice show an increase in the growth and in the sphingomyelin degradation rate. This suggests a cross-talk between connexin43 and the sphingolipid metabolism. It was not possible to assess a possible physical interaction between connexin43 and sphingomielinase since the expression of mutated forms of connexin43 is cytotoxic for C2C12 myoblasts. Abstract 2: Gold nanoparticles (AuNPs) can be used in biology in many ways, such as drug delivers. At the present time AuNPs has not been widely investigated using molecular methods such as magnetic resonance. The aim of this study is to identify suitable probes for the molecular characterization of AuNPs through electron spin resonance (ESR), making a wide screening of different types of probes: cationic, anionic and neutral nitroxide probes, with long or short tales, and nitronyl nitroxide probes with a thioether group. The results do not lead to the identification of any suitable probe to use in classical continuous-wave ESR studies. Most part of the registered ESR spectra suggests that the probes have remained in a rapid motion state as if they do not interact with the AuNPs. NNO-Benz and NNO-Al form a precipitate. NNO-benz and the long chain probes causes partial aggregation of the nanoparticles, showing a possible interaction with the particles. This could suggest that the probes remain in a rapid motion state even interacting with AuNPs, or that the unpaired electron of probes interacts with the plasmons on the surface of the metal, making the bound probes ESR silent. Further studies are required before making conclusions.
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