Bioquímico

I think is better to talk about disciplines rather than science or not-science. Physics joint all disciplines, all of them relapse on physics sooner or later, then chemistry and, as a high specialized chemistry, biology are the three major disciplines. These three are the big science fields, because they use the scientific method. Engineering, geology even medical sciences and others are applications of the three big sciences, so they still are, but not by them self. Mathematics does not seem like a science for me, it is the most amazing creation of human mind, mathematical modeling is the alphabet of science, but is in other dimension. PS. sorry for my English expression skils

I think this is the right site to place my question. I am adapting the method performed by El Mernissi and Doucet on “Quantitation of [3H]ouabain binding and turnover of Na-K-ATPase along the rabbit nephron” in order to do ouabaing binding on tubule suspensions. There is one useful equation that I have no idea where it came from. “Assuming that the concentration of free ligand remains constant throughout incubation (…) it is possible to determine k1 and k-1 using the standard equation: Log ( [EL]eq / [EL]eq - [EL] ) = (k1 [L] + k-1) t Where [EL]eq and [EL] represent the concentration of the enzyme-ligand complex at equilibrium and at time t respectively, and [L] is the concentration of free ligand.” I am wondering if anyone could expand this. Thanks in advance. PS: how did you write those equations, I mean how to write in chemical format. Thanks.

Hi, my name is Agustín; I am a biochemist, I have got my degree in the University of Buenos Aires, Argentina. Now I am doing research in the United States of America in physiology, specifically in activity and enzymatic parameters of Sodium-Potassium-Adenosinetriphosphatase in the kidney on a rat model of hypertension. My goal here is to get data and write my Doctoral Thesis to be presented in my home country. I hope this site will be an interesting source of knowledge sharing. It is a pleasure to be here.