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Posts posted by Ema
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Thank you so much for taking the time to answer so thoughtfully! This is really helpful to me. I wish you all the best.
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Hello :)
If I have plasmid DNA that I want bacteria to pick up, how should I encapsulate it to make it attractive for them? At the same time, I want the capsule to be immuno-invisible to the human system.
Do you know of any particular techniques? Possibly companies/researchers who focus on this?
Bacteria exchange the plasmids between each other, but would they also pick them up from the environment, if the plasmids were, say, encapsulated in a micelle?
Curious to hear your knowledge and thoughts. Thank you!
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Plasmid delivery
in Biology
Posted
@Dagl1 The protein on the plasmid should ideally only be induced by a promotor when needed, but until then it should preferably be immunoinvisible - while not producing the protein. I was hoping there could be an encapsulation made out of e.g. micelle membrane that would encapsulate it, be non-immunogenic itself and the plasmid would only be activated once fused with a cell that can produce it. The problem is, how to find such a capsule and make the cells take it up even from these 'plasmid vesicles'.
Also, @CharonY, I only signed in yesterday and didn't know you could tag people in replies. So thank you both for sharing your ideas.