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Ema

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About Ema

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    Lepton

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  • Favorite Area of Science
    Biotechnology
  1. Ema

    Plasmid delivery

    @Dagl1 The protein on the plasmid should ideally only be induced by a promotor when needed, but until then it should preferably be immunoinvisible - while not producing the protein. I was hoping there could be an encapsulation made out of e.g. micelle membrane that would encapsulate it, be non-immunogenic itself and the plasmid would only be activated once fused with a cell that can produce it. The problem is, how to find such a capsule and make the cells take it up even from these 'plasmid vesicles'. Also, @CharonY, I only signed in yesterday and didn't know you could tag people in replies. So thank you both for sharing your ideas.
  2. Ema

    Plasmid delivery

    Thank you so much for taking the time to answer so thoughtfully! This is really helpful to me. I wish you all the best.
  3. Ema

    Plasmid delivery

    Hello :) If I have plasmid DNA that I want bacteria to pick up, how should I encapsulate it to make it attractive for them? At the same time, I want the capsule to be immuno-invisible to the human system. Do you know of any particular techniques? Possibly companies/researchers who focus on this? Bacteria exchange the plasmids between each other, but would they also pick them up from the environment, if the plasmids were, say, encapsulated in a micelle? Curious to hear your knowledge and thoughts. Thank you!
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