Biochemistry and Molecular Biology

Hypothesis #42 This hypothesis is designed to suggest how scanning tunneling microscope technology combined with the knowledge of the atomic structure of DNA could be used to create life from atoms in there free form. Note that this hypothesis is designed to suggest the possibility of such an experiment, not to explain the detailed procedures that would be involved. There are also many assumptions being made about the current limits and abilities of the technologies available to today’s scientists. In recent years the technology of scanning tunneling microscopes has been used to arrange various atoms into rings, characters, and even three dimensional structures. …

Hello, I am a student at a community college, planning career in genetic engineering. Since I have never heard of any university having undergraduate degree in genetic engineering, what major would you suggest I chose that would bring me closer to my choice? I was thinking of biomedical engineering or human genetics. Or has anyone heard of dual program for these two majors?

Do you think it has significance?

Does anyone have some Thiobond resin (Invitrogen) left ? Invitrogen does not sell it anymore. Thanks

Suppose I attached magnetic particles to the rotating subunits of the ATP synthases. Then I'd set a rotating magnetic field around the subject. I guess it would start its rotation and synthesize ATP, or maybe not... A bigger problem would likely to be how to align the mitochondria, so all the synthases would rotate in the correct direction. Maybe use another field to align them from time to time. Also unless you had spesific magnetic particles inside your body, the rate of ATP synthesis would be kind of uniform.

In a science project.I'm trying to manipulate ants by isolating the pheromones.I need help on how i could do this.I also need information about the structure of pheromones.

Dear Colleagues, We are proud to announce the 2nd Horizons-Meeting entitled "Decoding Nature: Hierarchy of Interactions". The meeting will be held at the Max-Planck Institute for Biophysical Chemistry in Göttingen, Germany on 17th - 19th of March 2005. Our meeting aims to provide deep insights into contemporary life science research and to encourage exchange among people from different biological science branches. We invite distinguished scientists from fields as diverse as structural biology, developmental biology, cell biology and neurobiology. By including such a diverse spectrum of topics we hope to broaden horizons and to inspire interdisciplinary discussions. T…

I want to do a school project about ants.I wish to develop a chemical that could either mimic or change the chemical structure of the pheromones secreted by the queen ant,therefore allowing me to control the colony.Recently,i have analysed a local ant warder/killer and found out that it contains sulphides and sulfur.I have try mixing sucrose with the above chamicals to test the ants' reaction.However,i hit upon a paradox.the ants seems to be both attracted and warded by the mixture.I used a mxiture containing 4 parts sugar and 1 part sulfur.can any1 plz explain why?Btw,does anybody got any ideas to control the ants via chemicals?

where can we get the full length of amino acid sequences? i have very little bioinformatic background so please the more you explain the better it would be. thank you!

hello everybody! i wonder what can i do to get my nucleotide sequence and aa of a gene being translated under each other so i can have a whole picture of which nucleotide belongs to the aa and which aa belongs to the nucleotide in a paper? for example: atg ttt tta ........ M P L ........... this would be of great help, since the aa and protein sequence of a gene are in different places (databanks and even in the same databank) and i waste my time on translate every code into its aa. hope you get my point! thanks alot!

Hey! I'm preparing to write a science fiction novel. A central part of it involves a race of humans genetically altered to breathe carbon dioxide instead of oxygen so they could live on planets like Venus. I'm curious as to what conjectures, ideas, or theories any of you could present on how CO2 would affect the body differently than oxygen. Would it affect skin color? blood color? how long they could hold their breath? I'm not interested in whether it's possible or not. After all, this is science fiction. But assuming it was possible, what would it be like? Any ideas, from the mundane to the far-fetched, will be very appreciated. Thanks Josh

hi guys! what types of cells is a mammalian cell? i know this is maybe a "horrible" question, but i find it hard knowing the different cells under this category cell. i have looked in the net, but don't find any site where they explain about this type of cell. so hope for some ideas. thanks!

hi guys! how can i determine the hybridisation's temperature empirically? is there a website for doing this or any tricks? hopes for replies! thanks alot!

Hey, Was wondering if anyone had any knowledge that could help me sort out a problem with an ELISA I'm working on. I'm using a savinase sandwich ELISA, with a BSA blocker, and H2O2 / Orthophenylene Diamene Dihydrochloride to colour the substrate. I'm recieving very high absorbance readings which is making my results difficult to analyse. I have also used this method to test for puradax, termamyl and alcalase with no excessive background. Does anyone have any ideas as to why savinase is the only one with high background, or any ideas as to how the problme might be remedied? Thanks for any help you could give me Nate

As this is my first post i would like to say hello to the science community! As for the topic,I was planning on researching plant DNA and Biology, and i have found some interesting informational websites. I have narrowed down my interests to the study of plants, their composition and DNA and it would be great if you, the science community could share some possible experiment ideas for this topic. Any ideas will be appreciated. If this post is not detailed enough due to my extremely busy schedule, i apologize. Thank you!

Hello everyone, My name is Ricky Shadrach, currently a student of computer science at Virginia tech. I mention this only so that you get the impression that I have absolutely no background in biochemistry and hoping that your replies are made as, well, easy to follow as possible. Anyways, I normally post at the Skeptic Friends Network (http://www.skepticfriends.org), but have also for the past few months have been posting at http://www.skeptictimes.com (ignore the name, it is a Creationist website). At their forum, a user claimed that something called "ccdi9" completely refuted gradual evolution (who also claimed to be close to a PhD in biochemisty at age 21). …

wrong forum!

chromatography: we have to collect the fractions from the column. i wonder what is the difference between; flowthrough fraction, wash fraction and eluate fraction? i think i know what it means with eluate fraction, but what about the other two? hope for replies! thanks a bunch!

is bed length the heigh of the column or is that the heigh of the matrix/gel? i don't know where to put this question, but since it is about proteins then i guess here is the place thanks!

what is the difference between a non-reducing and a reducing buffer? :uhh: and what does it mean with non-reducing and reducing? thanks a bunch!

hello guys! I need to prepare a 2 mg/mL DNAase solution. the DNAase stock has a concentration of 2U/ml. how can i make a 2mg/ml from a 2U/ml solution? this is quite hard to figure out hope very much for replies! thanks for helping!

anybody have an image of the 5HT1 receptor? i need to know the structure of it ASAP. if you find it, i would be most grateful. thank you.

I know it would be extremely difficult in practice if not impossible but could an animal (in theory) be modified to breathe fire as an attack or defence? If the animal could be modified to produce a flammable organic liquid in a gland in its mouth and have an 'ignition spark' (generated in a similar way to how the electric eel makes electricity) at the opening of its mouth, then could it not create an aerosol of fuel and ignite it when provoked?

if i insert lacZ into a gene and the translation gives a hybrid product with the sequences of both lacZ and this gene. i wonder if lacZ can still work and give blue colonies? and what about the function of this other gene? wait so much for replies. thanks so much in advance!

all i learnt about protein expression till now is gone, may i say, just because an explanation from my professor...i just hope that he was not right about this, since it turns my "world" up side down! the aim was to investigate the different expression degrees of the yeast genes. we used S. cerevisiae. we transformed the yeasts with a transposon carrying a lacZ gene. by homolog recombination the transposon with the lacZ gene was inserted into the different yeast genes. we selected for blue colonies. we got different degree of the blue colour. it showed that we got less blue colonies than expected. i asked my professor and he explained; -> because 80% of the yea…