Biochemistry and Molecular Biology

Hey. So I was a bit of an idiot and forgot to digest away my template after transcription. Yeah figures. So as I've already added loading dye to my transcrption I can't exactley add DNase I to it now, so I got to run it on a gel first. I guess my quesiton is: is there a significant enough difference in the migration rates of DNA and RNA on denaturing PAGE (urea) that I will be able to differentiate two peices of about equal length. Lets say the RNA is 350 bp and the DNA 22 bp longer than that. If I do get two bands differentiating between the two shouldn't be difficult since only my RNA is radio-labled. Some one please say yes. I really don't want to have to…

I am not sure if this is the correct section to post this, but anyhow I got a question. How do you identify the amount of calories in foods that are not necessarily canned, but rather cooked at home or bought from a restaurant?

Do the constitute everything chemically in regards to biological systems, such as any enzyme recorded or protein for instance? I never resolved this question for some reason.

Hello !! I want to know the machanism of the protein labeling with antibodies. I want to label a protein that is being synthesized in the cell. I don't understand, how this thing is happening... How the antibodies pass the selective membrane?? Thank You.

I know that the human body has around 47 elements. I can't seem to find out what elements or how many are in primates. If anyone could tell me or lead me in the right direction that would be great.

New version NOC-3.0 is released for Windows/Linux/Mac OSX/FreeBSD/Solaris with source code. Downloading is available at http://noch.sourceforge.net NOC is: a easy and fast protein explorer for structure visulization, analysis; a powerful tool for crystallographic mapping, modeling and refinement; a efficient viwer for GROMACS/AMBER MD trajectories;

Hi all, Hope this is the right place to put this! I'm a little confused as to the results of an experiment I have just completed. It involved taking vitamin C or E or a mixture of both and then measuring RQ, O2 consumption and CO2 production. On the week of taking the vitamin E tablets, the RQ dropped dramatically as the O2 consumption increased. Any ideas as to why this happened? Also, in the following two weeks (one of these weeks was taking vitamin C and E tablets together, and the final week was taking neither) the RQ went back to as it had been previously, but overall, the O2 consumption and CO2 production both had been reduced by a large margin. I'm …

What are the differences between Biochemistry, Chemical Biology, Molecular Biology, Genetics, Biological Engineering and Biochemical Engineering?

Hello again I was talking about the comets that you get when treating DNA with factors that cut the DNA molecule, you start the electrophoresis and then on fluorescent microscope you see the fragments that have been cut. http://library.wolfram.com/examples/cometassay/Images/index_gr_6.gif Hello, I am characterising comet tails. I am not using a specific software for that, just Photoshop. I got problems cause when calculating the lenght of the comet tail, there are some fragments that are far away from ... how to say it... the main tail, that is intensively green. What should I do? Should I include them in the lenght of the whole comet? I am doing this for the fi…

Let say you fragment genomic DNA into around 500bp fragments. Now lets say you denature these fragments by heat or whatever. How would you go about ensuring that as many fragments as possible anneal to their complementary strand. Now obviously as you'll likely have 1000s of times genomic coverage each fragment will have 1000s available that it can consider complementary but it will have about 10 000 - 100 000 times that are not considered complementary. Conventional wisdom suggest that the best solution is to just mix them together in a buffer of moderate salt concentration heat a beaker to around 95C drop the tube containing the oligos in and let it slowly cool to…

Does anyone know of any websites on preparing slide, stain, and handling slide and stuff like that?

Principles of Gel Permeation Chromatography present the principles of gel permeation chromatography (GPC) for students in introductory undergraduate courses of chemistry and biochemistry. These principles are presented in four sections: Introduction, Real Lab, Virtual Lab, and Microscopic Model (Figure 1). The Introduction and Real Lab sections present a brief view of the basic experimental apparatus typically used in laboratory GPC in order to provide a concrete connection of the real process of separation. The basic elements of column chromatography, emphasizing the stationary and mobile phases, are presented in the Introduction, followed by a sequence of pictures …

Does anyone know if sodium p-hydroxymercuribenzoate is a competitive or non competitive inhibitor of leucine amino peptidase?

Hi Could someone please help me by explaining what the conjugated secondary antibody Anti mouce HRP is ? I mean why is it conjugated with HRP and not something else for example FITC? Thanks!

Hello everybody, I'm writing an essay about "Myoadenylate deaminase deficiency" for my Molecular Biology class. I looked up for it on the net, but what I could find was a short and incomplete explanation on Wikipedia with no figures and a few articles in which the disease was mentioned with no real focus on it and no details. What I'm looking for is: - Summarizing the clinical manifestations of the disease - The gene responsible and its mutations responsible for the disease - The normal and aberrant functioning of the protein product and some interesting facts and a few figures (like the AMP deaminase enzyme, a histology slide showing the effect …

i'm currently writing a piece of c++ code for improved primer selection for our lab. nothing fancy really. lately i was annoyed by the incorrect melting temperature calculated by 4+2 rule therefore i upgraded to nearest neighbour method. i read the papers by breslauer, santalucia, sugimoto and the rather crappy one by panjkovich - but i'm not really into the subject matter. question is: why are there only ten watson crick interactions that all authors provide thermodynamic data for? They provide data for AA, AT, TA, CA, CT, GA, GT, CG, GC and GG (with complementary nucleotides) but not for AC, AG, CC, TC, TG, TT. I wouldn't really care, but since i want to calcula…

Is there any difference in regards to molecular biology and biochemistry? Could a person basically view them as the same field really overall.

Hi all, I require soem help in my experiment.. Did a few round already, cos the first round, my protein was not pure ie cross contamination between V8-GST adn GST protein. 2nd round was low yield.. 3rd round (the most recent) the protein sort of degraded a little bit. But I made sure not to cross contaminate by using loads of pipettes.. And I made sure to put my protein samples on ice all the time. And centrifuging at 4 degrees when i need to wash off excess protein bound to beads. What could cause all possible errors? Could soemone pls help with troubleshooting, ie suggest possible steps in which i could hv gone wrong?? these are the steps i really paid…

I really need to find out how to analyse starch concentrations quantitatively instead of the simple iodine test, which doesnt really give figures. Can anyone help?

I have produced polyclonal antibodies in the past to synthetic peptides... I have had varying degrees of success but have a few peptides that consistently give low titres when conjugated to KLH. It has been suggested that I perhaps use the peptides conjugated to two carrier proteins say KLH and then another.... the person who suggested it advised me to imunise using KLH then boost with the other peptide conjugated to the alternative carrier and then continue to alternate with each boost. This is something he has apparently read and the paper claimed to increase titre levels, he has not performed this method himself! has anyone got any experience or thoughts on thi…

I am sure many people in SFN has heard of Nootropics. Referred to as "smart drugs" and "smart nutrients", are substances which boost human cognitive abilities (the functions and capacities of the brain). Typically, nootropics are alleged to work by increasing the brain's supply of neurochemicals (neurotransmitters, enzymes, and hormones), by improving the brain's oxygen supply, or by stimulating nerve growth. Keeping the brain's neurotransmitters at high levels improves concentration, mental focus, calculation ability, memory encoding, recall, creativity, mood, and cures and prevents most depressions. So I was wondering has anyone here on SFN developed their own Nootropic…

This is something like the chicken and egg story, but it involves DNA. We know that DNA encodes for proteins and proteins are needed for the transcription of DNA. So, which one comes first...DNA or proteins? DNA cannot be transcribed into mRNA (and later translated into proteins) without proteins (RNA polymerases), and proteins cannot be formed without DNA? So, how did the first DNA produce the first protein? :confused:

Hi, I'm currently using Q-PCR to look at changes in gene expression under different experimental conditions. However, for one of the primers I'm using, I get a very noisy baseline. As expected, the noise increases as the primer concentration is reduced, but I don't understand why the noise is so high when the Ct value is still quite low. Are there any factors that contribute to a nosiy baseline? contaminations in the Q-PCR mixture perhaps? Any help would be greatly appreciated.

is it ok to store SDS-PAGE gels in ddIH20 overnight and possibly longer after staining?

Hi everyone, I'm learning alot this year about biological chemistry, and I've become very interested in the cytochromes. Can anyone recommend any books with an in-depth discussion of the cytochromes? Especialy cyt a and cyt c ? (any papers about the functions and structures of these two would also be welcome!) Matt