Biochemistry and Molecular Biology

I want to prepare 1mg/ml stock of γ-Globulins from bovine blood as a standard for Bradford's assay, I dont know if I should dissolve the powder in distill water or some buffer? Please guide me with the same. I also want to prepare 25U/ml Dnase I and add it in my lysis buffer to lyse proteins. I have a powder form of Dnase which mentions it is 20000 U and wt is 4.12g, I am confused about how to prepare a stock of 25U/ml and I should dissolve the enzyme in what type of solution? water or some buffer or glycerol???

Hello, In deltaG = deltaG' +RTlnK once at equilibrium deltaG = 0 so deltaG' = -RTlnK This is supposed to be at standard temp, ph and conentration but I see it often that people will find the Keq and sub it in and get deltaG' at whatever concentration and temp, and ph they are working with for their system. That is, they are not at standard conditions. Why is this so? Is just the matter of defining your own "standard" conditions? How then do you universally say this is deltaG for this enzyme and this enzyme and be sure that their conditions are the same?

I was looking over the mechanism of alpha amylase hydrolysis of starch. It seems that a particular pair of glucose monomers are held in place with hydrogen bonds by the amylase enzyme. The molecule bends due to these bonds thus lowering the energy required to cleave the glycosidic bond between them. My question is, what locations on the amylase enzyme and the starch polymer does the hydrogen bonding take place ? I found a representation of what goes on: It shows the starch held in place.... Where are these bonds made to keep the molecule in place ? I know its probably a stupid question because amylase looks like this but it would be interesting to kn…

We know that facial nerve is about 1.8 mm in CPA, and it is only 0.68 in labyrinthine section. I would like to know histological chnages in facial nerve that allow facial nerve to be compressed in this way.

Hello, I am looking for some kind of online table or conversion tool that shows the elemental percentage of chemical compounds such as vitamins, minerals, trace elements and amino acids. As I am not a biochemist, I do hope to have formulated the question understandably. Let me give a few examples: 1. How much elemental magnesium is in magnesiumcitrate dihydrate? 2. How much actual Vitamin B1 / Thiamine is in thiamine hydrochloride? 3. How much actual/elemental zinc is in zincsulphate monohydrate? So what I need is a table or tool that will show me actual "content" of all sorts of vitamin, mineral, trace element and amino acid compounds. Apprecia…

Hello. Perhaps someone can help or point in the right direction, I am lost and am not a studied biochemist... and have googled for hours without any real success: I am trying to figure out a way to help/stimulate the body recycle NADH to NAD+ with the help of micronutrient supplementation (vitamins, minerals, trace elements, amino acids and so forth). The aim would be to get a proper NADH/NAD+ ratio resp. to decrease a high NADH-level due to a lot of NAD+ being used up by increased enzymatic processes, with or without having supplemented with an NAD+ precursor. Anyone have any ideas? Appreciate any info! Alexander

Hi! I am working on different types of luciferase. I like to chosen the eukaryote cell for experssion and studying of this luciferase in these cells. Can anyone please help me with this? thankyou.

what do citrus plants/fruits use the acid for? can it be metabolized by the plant/fruit or an animal/person?

Hi, I am looking for someone who has the facilities to conduct a modified Luria-Delbruck fluctuating test. I am willing to pay for the same as a service. Please contact me through email (melanon@gmail.com) or by phone +91 9841119309. Please make note of the following requirements for the project. Minimum of 200 plates for parallel cultures Digital camera for high resolution images of cultures (need time to be recorded with the photographs) Thanks in advance Maurice S. Devaraj

Hello, I am trying to install Rosetta Commons on my Mac (Snow Leopard) and if anyone has done this please help me out if you will. Or if anyone has ever used the terminal to install any of these types of programs that require "Scons" and so forth please be in contact with me via private chat where we can exchange an email. Thank you

Hello everyone, well I'm performing some experiments using a new type of vector and for these I have to use Fusion PCR technique to get my genomic fragment into the vector, but I have been having troubles with these because my only Polymerase is the normal TaqPol haha, I think my problem lies in the Termocycler programing wich to be honest I dont know for this kind of PCR, I would really appreciate if someone could tell me the conditions needed for Fusion PCR with TaqPol, or some suggestions. Thank you. Greets

ATP is very efficient energy carrier in our cell system. Why can we use it as an energy carrier? Are there any other alternatives? The system has been tested about past 4.0 2.0 billion years.

i was wondering about plasmid cloning and why some plasmids enter to the cell (EcoRI for exemple) and others don't !!

Plants produce sugars for energy to grow through photosynthesis. Could that process be skipped by just adding those necessary sugars to a plants water supply allowing them to grow in the dark? Could these sugars be added to plants that get sunlight as a growth booster? Thanks

I have a question about the length of typical alpha-helix transmembrane domain of membrane proteins. It is known that normally transmembrane alpha-helixes consist of 20-25 aminoacids and have the length about 3,5 nm to perforate the membrane. But what happens if the transmembrane domain is longer than 25 aa? I can't find any information about this problem, maybe here I'll find someone to help me?

Does anyone know why one would do a slant vs. a petri dish type of media setup?

Is it true that you can die from a 'broken heart'? Has anyone ever died as a result of a broken heart? (e.g. after the death of a loved one)

Hello, I have phase separation problems after homogenization and chloroform addition. Some of the samples have a very thick interphase and little/no aqueous phase after centrifugation and once inverted aqueous phase. What will be the cause of this situation and how should I overcome this?

Hello, I am working with the mentioned species and I am trying to isolate the genomic DNA. This species makes spores after some time but overall spores form throughout the growth period. Does anyone know a good strategy for isolating genomic DNA from spores? Or have experience with working with sporulating streptomyces and isolating their DNA? Thank you.

Hello all, I am a current Biochemistry Graduate Student and need to pick a couple of topics to write research proposals on using the NIH research proposal format. I came on this forum because I have been searching for a couple of weeks now and I haven't found anything that interesting. I need some topics which that are current and could maybe provide up to 2 years of research. If you have any ideas or have seen any interesting journal articles I would appreciate it! Thank you. (Also, these topics would not be for my current research, we are supposed to write hypothetical research proposals so they can be on any topic.)

Dear Colleagues, I need a reference (for the article "Steady-state physiology…" I have just written) that proves the pumping role of Na,K-ATPase. I have tried to locate any publication that demonstrates the pumping function of Na,K-ATPase, but have found nothing. Please help me with references (even one would be enough). To make it clear what I need, let me to give the following clarification. We believe that the ionic asymmetry between the cell and its environment is provided by a plasma membrane pump, Na-K-ATPase. Because of this pump, the concentration of K+ in the cell is above that in the medium and the concentration of Na+ is lower than in the medium. The pu…

I'm a newbie of doing co-ip I always find it's very difficult to adjust similar co-transfected vs single transfected protein expression amount for co-ip several different constructs. (My boss's absolute requirement before actually doing co-IPs) like: Protein A + vec Protein B + vec Protein C + vec A+B (both upregulated) A+C (both upregulated, but A has much more upregulated level than A+C or A alone) So, the level of A will never be the same in all cases (A, A+B, A+C)! One of my labmates suggests me to simply mix single protein-transfected HEK293 lysates together. i.e. A-transfected lysate+ B-transfected lysate = A+B. Do it for all sorts of combinat…

I'm running a search on BLAST (NCBI) using a protein sequence from Streptococcus sp. Most of the hits I get are from the same genus and even species (more than 50 out of 100 total). Is there a way to obtain hits that represent a wider range of organisms. In other words, it is possible to filter BLAST results in order to show up only one sequence per species? All the best, Gianluca

Hi! I am working on different types of RNA. I heard that RNA subunit of telomerase is small nuclear RNA. Then what is the difference between small nuclear RNA and telomerase RNA?? and also the repeats that telomerase adds on, what is the termination codon in that case?? How does it stop from adding on the repeats? Can anyone please help me with this? thankyou. regards, samantha

what would happen if we put human sperm into a chicken or any other animal and vice-versa?