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Proteins with natural affinity with Ni2+


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#1 ScienceN00b

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Posted 6 January 2017 - 12:16 AM

Hello,

 

For my homework (prior to work in the laboratorium) I need to find out which proteins have a natural affinity with Ni2+. I've tried googling it but unfortunately I didn't find the answer...

 

Thanks! :D


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#2 northernlad2690

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Posted 6 January 2017 - 12:32 AM

im assuming you are looking at Nickel affinity chromatography.


Edited by northernlad2690, 6 January 2017 - 12:41 AM.

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#3 ScienceN00b

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Posted 6 January 2017 - 01:04 AM

We'll work on the purification of a recombinant protein from E. coli, we'll be using his6 tags and some assays (including Bradford) but no chromatography :)


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#4 ecoli

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Posted 6 January 2017 - 03:29 AM

Histidine is an amino acid with natural affinity for nickel, so peptides with polyhistadine residues (i.e. introduced with the his tag). The wording of your question (what you mean by 'natural') is ambiguous.


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#5 CharonY

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Posted 6 January 2017 - 10:31 AM

Yeah, there are a lot of  proteins that can bind Ni specifically or non-specifically. Running a lysate through a column usually yields you a relatively complex mix of proteins, with your target hopefully being the most abundant fraction.


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#6 ScienceN00b

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Posted 7 January 2017 - 04:16 PM

Apparently I understood the question wrong, thanks for all the answers! The correct answer was ''proteins with a lot of histidine'' 


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