Sign in to follow this  
Followers 0
ScienceN00b

Proteins with natural affinity with Ni2+

6 posts in this topic

Hello,

 

For my homework (prior to work in the laboratorium) I need to find out which proteins have a natural affinity with Ni2+. I've tried googling it but unfortunately I didn't find the answer...

 

Thanks! :D

0

Share this post


Link to post
Share on other sites

Posted (edited)

im assuming you are looking at Nickel affinity chromatography.

Edited by northernlad2690
0

Share this post


Link to post
Share on other sites

We'll work on the purification of a recombinant protein from E. coli, we'll be using his6 tags and some assays (including Bradford) but no chromatography :)

0

Share this post


Link to post
Share on other sites

Histidine is an amino acid with natural affinity for nickel, so peptides with polyhistadine residues (i.e. introduced with the his tag). The wording of your question (what you mean by 'natural') is ambiguous.

0

Share this post


Link to post
Share on other sites

Yeah, there are a lot of proteins that can bind Ni specifically or non-specifically. Running a lysate through a column usually yields you a relatively complex mix of proteins, with your target hopefully being the most abundant fraction.

0

Share this post


Link to post
Share on other sites

Apparently I understood the question wrong, thanks for all the answers! The correct answer was ''proteins with a lot of histidine''

0

Share this post


Link to post
Share on other sites

Create an account or sign in to comment

You need to be a member in order to leave a comment

Create an account

Sign up for a new account in our community. It's easy!


Register a new account

Sign in

Already have an account? Sign in here.


Sign In Now
Sign in to follow this  
Followers 0