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Ultracentrifuge vs. centrifuge for membrane separation

allaroundanonymous
in Biology

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Does the type of [ultra]centrifuge you use matter? Basically, when trying to use the same protocol using a regular centrifuge, I got very loose and slanted "pellets" which were all but impossible to separate from the supernatant, even after centrifuging longer. I used RCF*time equivalent values to start with. Could it be that a similar protocol just can't be used with a regular centrifuge, or am I doing something wrong?

If the force of the same, i.e. you do not need the higher forces of an ultracentrifuge it should not matter. What could make a difference is if you have different vials.

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