Hello again,

 

I have extracted RNA and i am satisfied with the purity (260/280 ratio) and the yield. What is troubling me is the very low 260/230 ratio? Therefore, i wanted to know how important is the 260/230 ratio in RNA extraction. I will use the RNA for qPCR.

 

Yield 26-35 ng/ul and 260/280 ratio is between 1.9-2.2

 

If i want to re-precipitate the RNA should i use sodium acetate or Lithium chloride to do this?

 

Thanks

It depends on what you extracted and how. If you used Trizol, or are doing phenol extraction there is a good chance that that is the absorbing substance.

Other typical things include humic acids (soil and sediment), peptides, a number of aromats and urea on top off my head. For clean up I would existing lab protocols based on you extraction method. That makes it easier to compare issues within your lab for your particular type of samples.

It depends on what you extracted and how. If you used Trizol, or are doing phenol extraction there is a good chance that that is the absorbing substance.

Other typical things include humic acids (soil and sediment), peptides, a number of aromats and urea on top off my head. For clean up I would existing lab protocols based on you extraction method. That makes it easier to compare issues within your lab for your particular type of samples.

i am using a kit for extraction. Our lab doesn't have any clean up kit.

Your extraction protocol does have a cleanup step (typically precipitation with washing of the pellet or affinity based immobilization followed by washing). There is no optimal protocol for every sample, usually a lab adapts one or a few standardized protocols as the basis and make adjustments as necessary.