We know that 2D, traditional flask culture does not properly mimic the microenvironment of cells in vivo. The convenience of 2d culture has allowed decades of research and industry down a path which has already seen many publications relevant in 2D, yet not applicable to 3D.

 

Have you started culturing in 3D? If so what methods have you used? Hanging drop? Scaffolds? Bioreactors? A combination?

 

 

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Hi.

 

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Example of latest tissue engineering research using a 3D method. Atala and others at Wake Forest Institute utilize multiple methods for various studies: http://link.springer.com/article/10.1007/s10439-015-1298-3