rasen58 Posted February 7, 2014 Share Posted February 7, 2014 A yeast extract contains all the enzymes required for alcohol production. The extract is incubated under anaerobic conditions in 1 liter of media containing: 200 mM glucose, 20 mM ADP, 40 mM ATP, 2 mM NADH, 2mM NAD+, and 20 mM Pi (inorganic phosphates). What is the max amount of ethanol that can be produced in these conditions? I have no idea what to do at all for this problem. Is there an equation for ethanol production? Link to comment Share on other sites More sharing options...
CharonY Posted February 7, 2014 Share Posted February 7, 2014 Yes there is. You will have to look up the metabolic steps involved coming from glucose and ending with ethanol (there are variations, though, check the one for Saccharomyces). Then look at what you got and figure out what the limiting concentration is. That will determine the maximum yield. Link to comment Share on other sites More sharing options...
rasen58 Posted February 7, 2014 Author Share Posted February 7, 2014 I found 2 ATP + glucose + 4 ADP + 2 Pi + 2 NAD+ 2 ADP + 2 pyruvate + 4 ATP + 2 NADH Then I tried plugging in the numbers from the problem, but I'm not sure what to do after that Link to comment Share on other sites More sharing options...
CharonY Posted February 10, 2014 Share Posted February 10, 2014 That is the part for glycolysis. Now you need to find the reactions for ethanol fermentation. Link to comment Share on other sites More sharing options...
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now