Hello Researchers and Science Lovers,

 

My problem of today involves mitochondria. Specifically mitochondrial metabolism.

I have a compound that i believe disrupts mitochondrial metabolism. However im short of ideas at the moment. I could use oxygen electrode for some predictions but what about proteins..How can i study this "disruption" of metabolism studying proteins involved in it as well? What your suggestion for proteins to look? How can i know this compound disrupts mitochondrial metabolism?

 

Thanks everyone. I hope i get some answers.

In a naive approach I would look at proteome changes between treated and untreated cells. Assuming that the metabolic changes are restricted to mitochondria, one way is to isolate them (e.g. using density centrifugation), and then do MS-based whole proteome profiling (e.g. LC-MS/MS and/or 2DGE followed by MS).

Another option is a metabolom based analysis (e.g. GC/MS based), though I generally find proteomic interpretation more straightforward (a combination is also possible, but more costly, of course).