Is it possible to use/re-induce agrobacterium cultures that have been prepared almost a week back? What is the best possible way to preserve processed agrobacterium cultures for longer durations?

Yes. transfer a small bit of your culture to new media and it should grow. For longer durations, the best means of preservation would be to make glycerol stocks.

I agree with glycerol stocks. One thing to be careful about is that long-growing cultures (i.e. just inoculating from ongoing cultures) tend to accumulate mutations over time which can cause issues in experiments. And obviously plasmids and other mobile elements can get lost under non-selective conditions.

Edited January 30, 201412 yr by CharonY

Thanks guys! I wanted to know how best will the culture work after it has been re-induced in fresh media?