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help needed (DNA)

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1. The reaction catalysed by an endonuclease. Which bond is hydrolysed? What are the products?


2. The reaction catalysed by a 5’to 3’ exonuclease and 3’to 5’ exonuclease. ??


3. Denaturation of DNA. Denaturing agents. Which interactions are broken? Is the process reversible?

DNA can be denatured by heating, the first step of PCR is denature by heating. During the denaturing, the hydrogen bond between the two strand of DNA and until the two strands totally separate. DNA can renature by annealing.

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