Jump to content

Cell lysis with urea

Asha
in Biochemistry and Molecular Biology

Featured Replies

Would 8 M urea brake up E. coli cells? If you had them in a pellet, used enough 8 M urea and only resuspended it?

 

Urea is only a chaotrope and as such does little to degrade cell wall and membrane. Without further treatment the lysis rate would be very low and not suitable for most molecular biological applications.

Archived

This topic is now archived and is closed to further replies.

Go to topic listing

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.

Configure browser push notifications
Chrome (Android)
  1. Tap the lock icon next to the address bar.
  2. Tap Permissions → Notifications.
  3. Adjust your preference.
Chrome (Desktop)
  1. Click the padlock icon in the address bar.
  2. Select Site settings.
  3. Find Notifications and adjust your preference.