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Undefined bands in Gel

sdem1
in Biochemistry and Molecular Biology

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Hi, I've been trying to run gels for a series of PCRs to test primers. However
the bands so far have been curvy, slanted and very unclear. I then tested a 100bp and 1kb marker in a gel I made to check that the error is from the gel electrophoresis and not the PCR. A similar problem occured however, and I can't seem to figure out what is wrong.

I'll attach both pictures. The first gel was run at 80V and is a 3% gel. The second gel is also 3% and it was run at 90V. The third gel is 2% run at 100V.

If anyone has any ideas on what to fix that would be great.

Thanks!

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Edited by sdem1

Several of the pockets appear to be damaged (have to be careful when pulling the comb out and do not poke the gel when you load it), Considering the quality of the gels I would also renew the buffer and make doubly sure that the agarose was completely dissolved before pouring.

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