mohamed samir Posted March 9, 2013 Share Posted March 9, 2013 I usually got an melting curve with multipeak. Can anyone tell me what is the exact cause for this Link to comment Share on other sites More sharing options...
CharonY Posted March 10, 2013 Share Posted March 10, 2013 Usually means multiple products. Link to comment Share on other sites More sharing options...
gfritz Posted September 14, 2013 Share Posted September 14, 2013 If the Tm is around 75C, it's primer dimer... compare to your NTCs. If it is higher in Tm, mis-priming due to poor primer design. Increase annealing Ta or redesign primers. www.youtube.com/watch?v=4JBFpXVXhyw Link to comment Share on other sites More sharing options...
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