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How to predict speed of lysis in a red blood cell solution?


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Blood was added to 12 chemicals. I predicted that the chemicals with a higher molecular weight would lyse slower than those of a smaller molecular weight. My results disproved that. The numbers where all over the place. What factor allows you to predict what will lyse quicky and what will lyse slowly. Please explain in detail. Thank you

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There is not enough information. Cell lysis generally occurs due to destabilization of the membrane and different compounds may have different modes of action. Pure water can, for example, result in lysis of red blood cells. The effect is quite different from e.g. using detergent. Most certainly the MW only plays a minor role (unless you are discussing a specific class of lysis agents).

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