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Estimation of ferum level in saliva

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Hello,

 

I am a studen of Dental Faculty, during preparation for scientific project it turned out, that estimation of ferum level is saliva taken from several patients would be very interesting. Unfortunately i can not find any "available for university students" method of this procedure. Biochemical faculty promised to help us in reagents and laboratory work, but still we have no good method of evaluation. Can you suggest us good method for estimation of ferum level in saliva? Any information/previous studies/methods will be very helpful :)

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To be more precise: we are willing to better understand or to find a cause of very rare "pigmented plaque" on children's teeth. We suspect, that it may be partly caused by increased amount of iron in their saliva. That's why we need to know their level and compare it with control patients. That's why I'm asking about laboratory method (reagents, procedure) which we can suggest to our biochemical department :)

I hope now it's a bit more clear ;)

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