Alamelu Nachiyappan Posted August 15, 2012 Share Posted August 15, 2012 Hi, How long the protein samples( cell Lysate) can be stored in 4 degree? If its there at 4 degree for more than 12 hours won't the Proteins be degraded?Can it be still used for the experiments like WB? Alamelu Link to comment Share on other sites More sharing options...
CharonY Posted August 15, 2012 Share Posted August 15, 2012 If it is a raw lysate without any inhibitors and nothing that prevents proteolysis you will have significant degradation. It is usually best to store it e.g. after precipitation or freeze drying, if possible. Link to comment Share on other sites More sharing options...
Alamelu Nachiyappan Posted August 16, 2012 Author Share Posted August 16, 2012 If it is a raw lysate without any inhibitors and nothing that prevents proteolysis you will have significant degradation. It is usually best to store it e.g. after precipitation or freeze drying, if possible. thank you Link to comment Share on other sites More sharing options...
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